dulbecco s modified eagle s medium dmem (Beijing Solarbio Science)
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Dulbecco S Modified Eagle S Medium Dmem, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 11142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 11142 article reviews
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Modification:Article Title: Engineered dual-fluorescence functional nucleic acid-based CRISPR/Cas12a biosensor for label-free ratiometric detection of site-specific DNA methylation Article Snippet: LbaCas12a (Cpf1), 10 × NEB buffer r2.1, and AciI endonuclease were obtained from NEB (MA, USA). .. Reagents for Article Title: Achieving catalytic immunogenic modification via plasmonically powered nanogenerators to potentiate calreticulin exposure and antitumor immunity. Article Snippet: Proteins are central regulators of cellular function, and deliberate disruption of intracellular protein homeostasis represents a promising avenue for cancer immunotherapy.. Despite its efficiency, aldehyde-mediated protein carbonylation faces major hurdles in localized tumor targeting without systemic toxicity for immunotherapy.. This study proposed a therapeutic paradigm termed catalytic immunogenic modification, enabled by a plasmonically powered nanozyme system that programs protein modification in situ. Article Title: Hierarchical periosteum-bone composite scaffold with staged release of dexamethasone and endothelial cell derivatives for efficient critical-sized bone defect repair Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (USA). .. Phosphate-buffered saline (PBS), Article Title: Porphyrin‑manganese trioxide-doped chitosan hydrogel with self‑oxygen supply enhancing photodynamic therapy effect for antibacterial and anti-inflammatory of infected wounds. Article Snippet: To combat microbial infection and persistent inflammation of wounds, the increasing type and dosage of antibiotics in wound dressing results in the formation of drug-resistant bacteria, which seriously delays the healing of infected wounds.. Thus, to develop alternative antibacterial wound dressings independent of antibiotics is imperative.. In this work, we established a novel degradable hybrid nanohydrogel material (TAPP/Mn3O4@CSGA) with antibacterial and anti-inflammation effects by loading 5,10,15,20-tetrakis (4-aminophenyl)-21H,23Hporphine (TAPP) with PDT function and Mn3O4 with SOD and CAT-like activities in chitosan hydrogel (CS-GA). Article Title: An evaluation of Tetrastigma hemsleyanum leaves: Digestion, absorption and anti-inflammatory effects via an in vitro digestion-Caco-2-HT-29 model Article Snippet: Tetrastigma hemsleyanum leaves (THL), a traditional edible and medicinal perennial plant, are known for their diverse physiological activities, including demonstrated efficacy against ulcerative colitis in vivo.. This study investigated the digestion and absorption of bioactive components in THL using an integrated in vitro approach combining simulated digestion model with a Caco-2 cell monolayer model. Additionally, we assessed the antiinflammatory properties and mechanisms of the in vitro digested constituents of THL in a human colorectal adenocarcinoma cell line (HT-29 cell) inflammation model. Our findings revealed six components in the digested THL as absorbable and transportable by Caco-2 cells.. Despite a reduction in antioxidant capacity occurring during digestion, primarily due to the loss of phenolic and flavonoid compounds, the digested THL retained significant anti-inflammatory activity. Article Title: The Study of the Antitumor Effects and Molecular Mechanisms of Byakangelicol on HepG2 Cells. Article Snippet: Byakangelicol (BYA) belongs to coumarins, which have anti‐inflammatory and anti‐tumor pharmacological properties, but its inhibitory effect on liver cancer and its mechanism are still indistinct.. This study explored the mechanism of action by which BYA affects hepatocellular carcinoma (HCC).. Cell cytotoxic and apoptosis assay results showed that BYA effectively killed three kinds of HCC cells and induced mitochondrial pathway apoptosis of HepG2 cells. Article Title: Astragaloside IV mitigates myocardial ischemia/reperfusion injury by modulating the 14-3-3η/GPX4 axis, reducing mitochondrial dysfunction, ferroptosis, and apoptosis. Article Snippet: Background: Acute myocardial infarction (AMI) requires timely restoration of cardiac perfusion, which often leads to myocardial ischemia/reperfusion injury (MIRI).. Ferroptosis, apoptosis, and mitochondrial dysfunction play critical roles in this process.. Astragaloside IV (Ast) has been demonstrated to possess multiple biological functions, yet its protective mechanisms against MIRI remain unclear. Saline:Article Title: Hierarchical periosteum-bone composite scaffold with staged release of dexamethasone and endothelial cell derivatives for efficient critical-sized bone defect repair Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (USA). .. Phosphate-buffered saline (PBS), Article Title: An evaluation of Tetrastigma hemsleyanum leaves: Digestion, absorption and anti-inflammatory effects via an in vitro digestion-Caco-2-HT-29 model Article Snippet: Tetrastigma hemsleyanum leaves (THL), a traditional edible and medicinal perennial plant, are known for their diverse physiological activities, including demonstrated efficacy against ulcerative colitis in vivo.. This study investigated the digestion and absorption of bioactive components in THL using an integrated in vitro approach combining simulated digestion model with a Caco-2 cell monolayer model. Additionally, we assessed the antiinflammatory properties and mechanisms of the in vitro digested constituents of THL in a human colorectal adenocarcinoma cell line (HT-29 cell) inflammation model. Our findings revealed six components in the digested THL as absorbable and transportable by Caco-2 cells.. Despite a reduction in antioxidant capacity occurring during digestion, primarily due to the loss of phenolic and flavonoid compounds, the digested THL retained significant anti-inflammatory activity. CCK-8 Assay:Article Title: Hierarchical periosteum-bone composite scaffold with staged release of dexamethasone and endothelial cell derivatives for efficient critical-sized bone defect repair Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (USA). .. Phosphate-buffered saline (PBS), Staining:Article Title: Hierarchical periosteum-bone composite scaffold with staged release of dexamethasone and endothelial cell derivatives for efficient critical-sized bone defect repair Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (USA). .. Phosphate-buffered saline (PBS), Radio Immunoprecipitation:Article Title: Hierarchical periosteum-bone composite scaffold with staged release of dexamethasone and endothelial cell derivatives for efficient critical-sized bone defect repair Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (USA). .. Phosphate-buffered saline (PBS), Lysis:Article Title: Hierarchical periosteum-bone composite scaffold with staged release of dexamethasone and endothelial cell derivatives for efficient critical-sized bone defect repair Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (USA). .. Phosphate-buffered saline (PBS), Viscosity:Article Title: Porphyrin‑manganese trioxide-doped chitosan hydrogel with self‑oxygen supply enhancing photodynamic therapy effect for antibacterial and anti-inflammatory of infected wounds. Article Snippet: To combat microbial infection and persistent inflammation of wounds, the increasing type and dosage of antibiotics in wound dressing results in the formation of drug-resistant bacteria, which seriously delays the healing of infected wounds.. Thus, to develop alternative antibacterial wound dressings independent of antibiotics is imperative.. In this work, we established a novel degradable hybrid nanohydrogel material (TAPP/Mn3O4@CSGA) with antibacterial and anti-inflammation effects by loading 5,10,15,20-tetrakis (4-aminophenyl)-21H,23Hporphine (TAPP) with PDT function and Mn3O4 with SOD and CAT-like activities in chitosan hydrogel (CS-GA). Food & Beverages:Article Title: Porphyrin‑manganese trioxide-doped chitosan hydrogel with self‑oxygen supply enhancing photodynamic therapy effect for antibacterial and anti-inflammatory of infected wounds. Article Snippet: To combat microbial infection and persistent inflammation of wounds, the increasing type and dosage of antibiotics in wound dressing results in the formation of drug-resistant bacteria, which seriously delays the healing of infected wounds.. Thus, to develop alternative antibacterial wound dressings independent of antibiotics is imperative.. In this work, we established a novel degradable hybrid nanohydrogel material (TAPP/Mn3O4@CSGA) with antibacterial and anti-inflammation effects by loading 5,10,15,20-tetrakis (4-aminophenyl)-21H,23Hporphine (TAPP) with PDT function and Mn3O4 with SOD and CAT-like activities in chitosan hydrogel (CS-GA). MTT Assay:Article Title: Porphyrin‑manganese trioxide-doped chitosan hydrogel with self‑oxygen supply enhancing photodynamic therapy effect for antibacterial and anti-inflammatory of infected wounds. Article Snippet: To combat microbial infection and persistent inflammation of wounds, the increasing type and dosage of antibiotics in wound dressing results in the formation of drug-resistant bacteria, which seriously delays the healing of infected wounds.. Thus, to develop alternative antibacterial wound dressings independent of antibiotics is imperative.. In this work, we established a novel degradable hybrid nanohydrogel material (TAPP/Mn3O4@CSGA) with antibacterial and anti-inflammation effects by loading 5,10,15,20-tetrakis (4-aminophenyl)-21H,23Hporphine (TAPP) with PDT function and Mn3O4 with SOD and CAT-like activities in chitosan hydrogel (CS-GA). Cell Culture:Article Title: The Study of the Antitumor Effects and Molecular Mechanisms of Byakangelicol on HepG2 Cells. Article Snippet: Byakangelicol (BYA) belongs to coumarins, which have anti‐inflammatory and anti‐tumor pharmacological properties, but its inhibitory effect on liver cancer and its mechanism are still indistinct.. This study explored the mechanism of action by which BYA affects hepatocellular carcinoma (HCC).. Cell cytotoxic and apoptosis assay results showed that BYA effectively killed three kinds of HCC cells and induced mitochondrial pathway apoptosis of HepG2 cells. Article Title: Astragaloside IV mitigates myocardial ischemia/reperfusion injury by modulating the 14-3-3η/GPX4 axis, reducing mitochondrial dysfunction, ferroptosis, and apoptosis. Article Snippet: Background: Acute myocardial infarction (AMI) requires timely restoration of cardiac perfusion, which often leads to myocardial ischemia/reperfusion injury (MIRI).. Ferroptosis, apoptosis, and mitochondrial dysfunction play critical roles in this process.. Astragaloside IV (Ast) has been demonstrated to possess multiple biological functions, yet its protective mechanisms against MIRI remain unclear. |
